Generation and evaluation of the efficacy of rhesus monkey soluble cytotoxic T lymphocyte-associated antigen-4 in the allogeneic mixed lymphocyte reaction

Title
Generation and evaluation of the efficacy of rhesus monkey soluble cytotoxic T lymphocyte-associated antigen-4 in the allogeneic mixed lymphocyte reaction
Author(s)
최인호김정식[김정식]이한형[이한형]이석준[이석준]Manheum Na[Manheum Na]김상훈[김상훈]한지혜[한지혜]배준범[배준범]최상필[최상필]김상훈[김상훈]박정규[박정규]전태훈[전태훈]
Keywords
CELL RESPONSES; CTLA-4; BELATACEPT; MODELS; PIG
Issue Date
201212
Publisher
SPRINGER
Citation
BIOTECHNOLOGY LETTERS, v.34, no.12, pp.2191 - 2197
Abstract
Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4; CD152) is a transmembrane protein that is structurally similar to CD28. As CTLA-4 has a much higher binding affinity to B7 than CD28, several approaches using soluble CTLA-4 have been tried to down-regulate T cell activity by blocking the interaction between CD28 and B7. We constructed soluble rhesus monkey CTLA-4 immunoglobulin (CTLA-4Ig) containing a critical binding site to B7 combined with a constant Ig heavy chain region in a mammalian system. Flow cytometry analyses indicated that soluble rhesus monkey CTLA-4Ig bound to rhesus monkey CD86 (B7.2). Moreover, soluble rhesus monkey CTLA-4Ig more effectively blocked the rhesus monkey-rhesus monkey allogeneic mixed lymphocyte reaction compared with that of humans. These results indicate that soluble rhesus monkey CTLA-4Ig may be useful in preclinical trials in a rhesus monkey model.
URI
http://hdl.handle.net/YU.REPOSITORY/26686http://dx.doi.org/10.1007/s10529-012-1026-0
ISSN
0141-5492
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