Enzymeless determination of total sugar by luminol-tetrachloroaurate chemiluminescence on chip to analyze food samples

Title
Enzymeless determination of total sugar by luminol-tetrachloroaurate chemiluminescence on chip to analyze food samples
Author(s)
당중덩Al-Mahmnur Alam[Al-Mahmnur Alam]Mohammad Kamruzzaman[Mohammad Kamruzzaman]Sang Hak Lee[Sang Hak Lee]Young Ho Kim[Young Ho Kim]Gyu-Man Kim[Gyu-Man Kim]
Keywords
FLOW-INJECTION ANALYSIS; MICROFLUIDIC DEVICES; GOLD NANOPARTICLES; HYDROGEN-PEROXIDE; GLUCOSE; ELECTROCHEMILUMINESCENCE; POLY(DIMETHYLSILOXANE); ELECTROPHORESIS; NANOWIRES; FRUCTOSE
Issue Date
201212
Publisher
SPRINGER HEIDELBERG
Citation
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v.404, no.10, pp.3165 - 3173
Abstract
Chemiluminescence (CL) emission from luminol-tetrachloroaurate ([AuCl4](-)) system studied in presence of monosaccharide sugars such as glucose and fructose was investigated on a microfluidic chip fabricated by the soft lithography technique. CL emission from the luminol-[AuCl4](-) system at 430 nm was intensified remarkably by the catalytic activity of glucose and fructose at room temperature. Under optimized conditions, the CL emission intensity of the system was found to be linearly related to the concentration of the sugars. Based on this observation, nonenzymatic determination of total sugar (glucose, fructose, or hydrolyzable sucrose) was performed in a rapid and sensitive analytical method. The results revealed that the linearity ranged from 9 to 1,750 mu M for glucose and 80 to 1,750 mu M for fructose, with a limit of detection of 0.65 and 0.69 mu M, respectively. The relative standard deviations determined at 250 mu M based on six repetitive injections were 1.13 and 1.15 % for glucose and fructose, respectively. The developed method was successfully applied for determination of the total sugar concentration in food and beverages.
URI
http://hdl.handle.net/YU.REPOSITORY/26683http://dx.doi.org/10.1007/s00216-012-6429-1
ISSN
1618-2642
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공과대학 > 기계공학부 > Articles
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