Iris Nertschinskia Ethanol Extract Differentially Induces Cytotoxicity in Human Breast Cancer Cells Depending on AKT1/2 Activity

Title
Iris Nertschinskia Ethanol Extract Differentially Induces Cytotoxicity in Human Breast Cancer Cells Depending on AKT1/2 Activity
Author(s)
홍남주맹형건[맹형건]신재식[신재식]홍성우[홍성우]문재희[문재희]김진선[김진선]서영아[서영아]김은성[김은성]이영민[이영민]김예슬[김예슬]최은경[최은경]김인기[김인기]이석영[이석영]최동형[최동형]김태원[김태원]진동훈[진동훈]이왕재[이왕재]
Keywords
PROTEIN-KINASE; PHOSPHATIDYLINOSITOL 3-KINASE; PATHWAY; ACTIVATION; SURVIVAL; AKT/PKB; GROWTH
Issue Date
201212
Publisher
ASIAN PACIFIC ORGANIZATION CANCER PREVENTION
Citation
ASIAN PACIFIC JOURNAL OF CANCER PREVENTION, v.13, no.12, pp.6511 - 6516
Abstract
Recently, we reported that an ethanol extract of Iris nertschinskia induces p53-dependent apoptosis in the MCF7 human breast cancer cell line. However, the detailed mechanisms were not fully explored. Here, we demonstrate another aspect of the activity of I. nertschinskia in breast cancer cells. We compared the response to an ethanol extract of I. nertschinskia in two different human breast cancer cell lines, Hs578Tand MDA-MB231, respectively with relatively low and high AKT1/2 activity by trypan blue exclusion assay and FACS analysis. Knockdown of endogenous AKT1 or AKT2 in breast cancer cells by RNA interference determined the sensitivity to I. nertschinskia ethanol extract compared to control cells. The I. nertschinskia ethanol extract induced cell death in a manner that depended on the level of phosphorylated AKT1/2 protein and was associated with a significant increase in the sub-G1 cell population, indicative of apoptosis. Our results indicate that an ethanol extract of I. nertschinskia differentially induces cell death in breast cancer cells depending on their level of phosphorylated AKT1/2.
URI
http://hdl.handle.net/YU.REPOSITORY/26679http://dx.doi.org/10.7314/APJCP.2012.13.12.6511
ISSN
1513-7368
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생명공학부 > 생명공학부 > Articles
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