High throughput screening methods for omega-transaminases

Title
High throughput screening methods for omega-transaminases
Author(s)
샘매튜윤형돈신기영손민수
Keywords
UNNATURAL AMINO-ACID; CHIRAL AMINES; ASYMMETRIC-SYNTHESIS; KINETIC RESOLUTION; SUBSTRATE-SPECIFICITY; ALPHA-METHYLBENZYLAMINE; PRODUCT INHIBITION; DIRECTED EVOLUTION; PROTEIN FUNCTION; ACTIVE-SITE
Issue Date
201302
Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
Citation
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.18, no.1, pp.1 - 7
Abstract
Recently, omega-transaminases have been increasingly used to synthesize amine compounds by reductive amination of prochiral ketones which are of high pharmacological significance. However, the conventional methods for evaluating these enzymes are time consuming and have often been regarded as a bottle neck in developing these enzymes as industrial biocatalysts. In the past few years, several high throughput screening methods have been developed for fast evaluation and identification of omega-transaminase. This review summarizes the various methodologies developed for rapidly screening omega-transaminases.
URI
http://hdl.handle.net/YU.REPOSITORY/26602http://dx.doi.org/10.1007/s12257-012-0544-x
ISSN
1226-8372
Appears in Collections:
중앙도서관 > rims journal
생명공학부 > 생명공학부 > Articles
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