형질전환 토마토의 T-DNA 도입 특성 분석

Title
형질전환 토마토의 T-DNA 도입 특성 분석
Other Titles
T-DNA Integration Patterns in Transgenic Tomatoes with PATII Gene
Author(s)
안순영윤해근박효근[박효근]
Keywords
reverse primer (rp) PCR; thermal asymmetric interlaced (TAIL)-PCR; transferred DNA (T-DNA) repeats
Issue Date
201212
Publisher
한국국제농업개발학회
Citation
한국국제농업개발학회지, v.24, no.5, pp.591 - 597
Abstract
The repetitive integration of transgenes in transgenic tomatoes was investigated. Several independent transgenic lines of tomato were screened for possible T-DNA repeats using reverse primer PCR (rpPCR), which utilizes primer pairs oriented in opposite directions. The rpPCR results showed that the binding sites for primers 3 and 4 were missed and were truncated at T-DNA copies in the 3 transgenic lines, including T0-21, -26, and -34. To assess the distribution of inserts, 52 flanking sequences were amplified by thermal asymmetric interlaced (TAIL)-PCR. In the nucleotide sequence analysis results of 22 flanking regions, two showed higher than 90% homologys to centromeric region of chromosome 12, one flanking sequence showed 98% homology to tomato clone 135006R, and five flanking sequences showed 97% homology to tobacco chloroplast genome DNA. Twelve of the 22 flanking sequences showed various vector sequences and thirty flanking sequences showed no significant similarity to any released tomato genomic regions.
URI
http://hdl.handle.net/YU.REPOSITORY/26598
ISSN
1225-8504
Appears in Collections:
중앙도서관 > rims journal
자연자원대학 > 원예생명과학과 > Articles
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