Bioconversion of p-coumaric acid to p-hydroxystyrene using phenolic acid decarboxylase from B. amyloliquefaciens in biphasic reaction system

Title
Bioconversion of p-coumaric acid to p-hydroxystyrene using phenolic acid decarboxylase from B. amyloliquefaciens in biphasic reaction system
Author(s)
정다혜[정다혜]최원지[최원지]최권영[최권영]정은옥[정은옥]윤형돈Romas J. Kazlauskas[Romas J. Kazlauskas]김병기[김병기]
Keywords
RECOMBINANT ESCHERICHIA-COLI; BETA-KETOADIPATE PATHWAY; LACTOBACILLUS-PLANTARUM; SACCHAROMYCES-CEREVISIAE; TRANSCRIPTIONAL ANALYSIS; KINETIC RESOLUTION; CHIRAL AMINES; GENE CLONING; PURIFICATION; OVEREXPRESSION
Issue Date
201302
Publisher
SPRINGER
Citation
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.97, no.4, pp.1501 - 1511
Abstract
Phenolic acid decarboxylase (PAD) catalyzes the non-oxidative decarboxylation of p-coumaric acid (pCA) to p-hydroxystyrene (pHS). PAD from Bacillus amyloliquefaciens (BAPAD), which showed k (cat)/K (m) value for pCA (9.3 x 10(3) mM(-1) s(-1)), was found as the most active one using the "Subgrouping Automata" program and by comparing enzyme activity. However, the production of pHS of recombinant Escherichia coli harboring BAPAD showed only a 22.7 % conversion yield due to product inhibition. Based on the partition coefficient of pHS and biocompatibility of the cell, 1-octanol was selected for the biphasic reaction. The conversion yield increased up to 98.0 % and 0.83 g/h/g DCW productivity was achieved at 100 mM pCA using equal volume of 1-octanol as an organic solvent. In the optimized biphasic reactor, using a three volume ratio of 1-octanol to phosphate buffer phase (50 mM, pH 7.0), the recombinant E. coli produced pHS with a 88.7 % conversion yield and 1.34 g/h/g DCW productivity at 300 mM pCA.
URI
http://hdl.handle.net/YU.REPOSITORY/26465http://dx.doi.org/10.1007/s00253-012-4358-8
ISSN
0175-7598
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