Comparative proteomic analysis of plant responses to sound waves in Arabidopsis
- Comparative proteomic analysis of plant responses to sound waves in Arabidopsis
- 배한홍; 권영상[권영상]; 정미정[정미정]; 차재율[차재율]; 정성우[정성우]; 박수철[박수철]; 신성철[신성철]; 정우식[정우식]; 배동원[배동원]
- Arabidopsis; sound wave; proteomics; MALDITOF/TOF MS
- Issue Date
- Journal of Plant Biotechnology, v.39, no.4, pp.261 - 272
- Environmental factors greatly influence the growth,development, and even genetic characteristics of plants. The mechanisms by which sound influences plant growth,however, remain obscure. Previously, our group reported that several genes were differentially regulated by specific frequenciesof sound treatmentusing a sound-treated subtractive library. In this study, we used a proteomic approach to investigate plant responses to sound waves in Arabidopsis.
The plants were exposed to 250-Hz or 500-Hz sound waves,and total proteins were extracted from leaves 8 h and 24 h after treatment. Proteins extracted from leaves were subjected to 2-DE analysis. Thirty-eight spots were found to be differentially regulated in response to sound waves and were identified using MALDI-TOF MS and MALDI-TOF/TOF MS. The functions of the identified proteins were classified into photosynthesis, stress and defense, nitrogen metabolism,and carbohydrate metabolism. To the best of our knowledge,this is the first report on the analysis of protein changes in response to sound waves in Arabidopsis leaves. These findings provide a better understanding of the molecular basis of responses to sound waves in Arabidopsis.
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