Determination of Polar Secondary Metabolomes in Arabidopsis thaliana using High Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry

Title
Determination of Polar Secondary Metabolomes in Arabidopsis thaliana using High Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry
Author(s)
배한홍조영아[조영아]박세민[박세민]배동원[배동원]서온누리[서온누리]이지은[이지은]정성우[정성우]권영상[권영상]차재율[차재율]신성철[신성철]
Keywords
Defense response; LC-MS/MS; Polyphenol; Secondary metabolites; Sound Wave; Defense response; LC-MS/MS; Polyphenol; Secondary metabolites; Sound Wave
Issue Date
201212
Publisher
경상대학교 농업생명과학연구원
Citation
농업생명과학연구, v.46, no.6, pp.165 - 171
Abstract
As a preceding study for investigating the influence of sound wave stimulus on Arabidopsis thaliana metabolomics, the polar secondary metabolomes of the plant were determined using high performance liquid chromatography coupled with tandem mass spectrometry. A total of 10 polar secondary metabolomes were characterized and quantified. Among them, 4 metabolomes, p-coumaroylagmatine isomer (7 and 8), p-coumaroylagmatine isomer (9 and 10) were identified in the plant for the first time. The validation was conducted in terms of linearity, recovery, precision, limit of detection (LOD) and limit of quantification (LOQ). The validated method was applied to the simultaneous quantification of the 10 polar secondary metabolomes.
URI
http://hdl.handle.net/YU.REPOSITORY/26353
ISSN
1598-5504
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생명공학부 > 생명공학부 > Articles
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