The effects of 8-arm-PEG-catechol/heparin shielding system and immunosuppressive drug, FK506 on the survival of intraportally allotransplanted islets

Title
The effects of 8-arm-PEG-catechol/heparin shielding system and immunosuppressive drug, FK506 on the survival of intraportally allotransplanted islets
Author(s)
정지헌임복현[임복현]Muhammad R. Haque[Muhammad R. Haque]이동윤[이동윤]안철희[안철희]김주은[김주은]변영로[변영로]
Keywords
MEDIATED INFLAMMATORY REACTION; HUMAN PANCREATIC-ISLETS; TISSUE FACTOR; SURFACE MODIFICATION; TRANSPLANTED ISLETS; ALLOGRAFT SURVIVAL; HEPARIN; XENOTRANSPLANTATION; INHIBITION; THERAPY
Issue Date
201303
Publisher
ELSEVIER SCI LTD
Citation
BIOMATERIALS, v.34, no.8, pp.2098 - 2106
Abstract
This study proposed a double-layer shielding method of using 8-arm-PEG-catechol (PEG(8)) and N-hydroxysuccinimidyl-linked unfractionated heparin (UFH-NHS) for the prevention of instant blood-mediated inflammatory reaction (IBMIR) and immune reactions against transplanted pancreatic islets. The surface of islet was evenly covered by PEG(8) and UFH-NHS. Both viability and functionality of islets were evaluated in vitro, and the anti-coagulation effect of conjugated heparin on the islet surface was also evaluated. The inhibition effects of PEG(8)/UFH double-layer shielding system on immune reactions and IBMIR induced by transplanted islets were evaluated in an allograft model. When pancreatic islets of Sprague Dawley (SD) rats were transplanted in the liver of F344 rats, the mean survival time (MST) of PEG(8)/UFH double-layer shielded islets (6.8 +/- 1.6 days) was statistically increased, compared to that of unmodified islets (3.6 +/- 1.1 days). Furthermore, when 0.5 mg/kg of FK506 was daily administered, the MST of double-layer shielded islet (15.0 +/- 2.1 days) was increased by two-fold, compared to that of unmodified islets treated with the same dose of FK506 (8.0 +/- 2.4 days). Therefore, a newly developed strategy of combining the PEG(8)/UFH double-layer shielding system with FK506 would certainly be effective for preventing immune activation and IBMIR against allotransplanted islets. (C) 2012 Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/26261http://dx.doi.org/10.1016/j.biomaterials.2012.11.028
ISSN
0142-9612
Appears in Collections:
약학대학 > 약학부 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE