Macroporous matrix for cartilage tissue engineering

Title
Macroporous matrix for cartilage tissue engineering
Author(s)
한성수딥티싱조선미돌리싱
Keywords
GENE-EXPRESSION; CHONDROCYTES; PROLIFERATION
Issue Date
201303
Publisher
INDIAN ACAD SCIENCES
Citation
CURRENT SCIENCE, v.104, no.5, pp.622 - 626
Abstract
Articular cartilage matrix experiences substantial structural, mechanical and molecular changes with ageing, which include surface fibrillation, variation in proteoglycan structure and composition, increased cross-linking of collagen and decreased stiffness along with tensile strength. Altered functionality of chondrocytes leads to changed extracellular matrix (ECM) functionality and to the development of osteoarthritis. Autologus chondrocytes implantation, the only feasible treatment, relies on the delivery of cultured cells. The purpose of our study was to isolate and examine microenvironment required to maintain the cellular phenotype of cartilage cells. Chondrocytes were isolated from full-thickness articular cartilage of the femoral condyles of skeletally mature bovines by sequential enzymatic digestion. Processed cells were cultured in different systems such as tissue culture plates (2D) and three-dimensional (3D) porous collagen matrix and characterized using cell surface antibodies, biochemical analysis and immunohistochemistry along with viability testing. The chondrocytes native matrix is composed of collagen II and proteoglycan macromolecules. Metabolic activity was found high among cells cultured in 3D collagen matrix and high ECM deposition was noticed. Further confirmation done by S-100 protein and various stains indicated that chondrocytes cultured on three-dimensional matrix retained the cellular phenotypes for longer duration and were highly metabolically active in comparison to the 2D control system.
URI
http://hdl.handle.net/YU.REPOSITORY/26222
ISSN
0011-3891
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공과대학 > 화학공학부 > Articles
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