Quantitative analysis of bioactive marker compounds from Cinnamomi Ramulus and Cinnamomi Cortex by HPLC-UV

Title
Quantitative analysis of bioactive marker compounds from Cinnamomi Ramulus and Cinnamomi Cortex by HPLC-UV
Author(s)
손종근정수양[정수양]Bing Tian Zhao[Bing Tian Zhao]문동철[문동철]강종성[강종성]이제현[이제현]민병선[민병선]우미희[우미희]
Keywords
acetonitrile; biological marker; cinnamaldehyde; cinnamic acid; Cinnamomum cassia extract; coumarin; water; article; cinnamon; controlled study; flow rate; process development; process optimization; quality control; quantitative analysis; reversed phase high performance liquid chromatography; separation technique; ultraviolet radiation
Issue Date
201303
Citation
Natural Product Sciences, v.19, no.1, pp.28 - 35
Abstract
In this study, quantitative and pattern recognition analysis for the quality evaluation of Cinnamomi Ramulus and Cinnamomi Cortex using HPLC/UV was developed. For quantitative analysis, three major bioactive compounds were determined. The separation conditions employed for HPLC/UV were optimized using an ODS C18 column (250 �� 4.6 mm, 5 ��m) with gradient conditions of acetonitrile and water as the mobile phase, at a flow rate of 1.0 mL/min and a detection wavelength of 265 nm. This method was fully validated with respect to linearity, accuracy, precision, recovery, and robustness. The HPLC/UV method was applied successfully to the quantification of three major compounds in the extract of Cinnamomi Ramulus and Cinnamomi Cortex. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of thirty eight Cinnamomi Ramulus and thirty five Cinnamomi Cortex samples. The results indicate that the established HPLC/ UV method is suitable for quantitative analysis.
URI
http://hdl.handle.net/YU.REPOSITORY/26194
ISSN
1226-3907
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약학대학 > 약학부 > Articles
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