Elimination of Chrysanthemum stunt viroid (CSVd) from meristem tip culture combined with prolonged cold treatment

Title
Elimination of Chrysanthemum stunt viroid (CSVd) from meristem tip culture combined with prolonged cold treatment
Author(s)
박경일Wina Dian Savitri[Wina Dian Savitri]전수민 [전수민 ]정미영[정미영]한증술[한증술]김창길[김창길]
Keywords
SHOOT APICAL MERISTEM; PLANTS; TRANSMISSION
Issue Date
201304
Publisher
KOREAN SOC HORTICULTURAL SCIENCE
Citation
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY, v.54, no.2, pp.177 - 182
Abstract
Chrysanthemum production in Korea has recently been greatly affected by the spread of Chrysanthemum stunt viroid (CSVd) infection, necessitating the use of CSVd-free stocks to ensure successful chrysanthemum cultivation. We investigated the effects of low temperature (4A degrees C), antiviral chemicals (ribavirin and amantadine) and a combination of these treatments on CSVd elimination by meristem tip cultures using plantlets that originated from CSVd-infected chrysanthemum 'Ency'. Neither antiviral agents led to CSVd elimination, despite the suppression of meristem tip growth in a concentration dependent manner. However, the CSVd elimination rate increased up to 42.8% when meristem tips were excised after storage at 4A degrees C for two months. The most effective results were obtained from a combination of low temperature for three months at 4A degrees C followed by meristem tip culture on media containing 50 and 100 mg center dot L-1 ribavirin. These results suggest that antiviral agents can also be useful for CSVd elimination if their treatment is combined with prolonged periods of low temperature. This is the first report of eradication of viroids from spray type chrysanthemum bred in Korea.
URI
http://hdl.handle.net/YU.REPOSITORY/26099http://dx.doi.org/10.1007/s13580-013-0141-8
ISSN
2211-3452
Appears in Collections:
자연자원대학 > 원예생명과학과 > Articles
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