Crystallization and preliminary X-ray crystallographic studies of the coiled-coil domain of PIST

Title
Crystallization and preliminary X-ray crystallographic studies of the coiled-coil domain of PIST
Author(s)
박현호서은경신영철[신영철]전주홍[전주홍]
Keywords
GOLGI PROTEIN; TRANSPORT; ASSOCIATION; PARTNER; COMPLEX; GTPASES; KINASE; RAB6
Issue Date
201304
Publisher
WILEY-BLACKWELL
Citation
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, v.69, pp.468 - 471
Abstract
PIST [PDZ ( PSD-95, Discs-large and ZO-1) protein interacting specifically with TC10] functions as a regulator of membrane trafficking with Rab6A. Recently, the involvement of the fusion of PIST with ROS1 in cancer development has been identified. In this study, the coiled-coil domain of PIST, which is the domain responsible for interaction with Rab6A and fusion with ROS1, corresponding to amino acids 29-133, was overexpressed in Escherichia coli using engineered C-terminal His tags. The coiled-coil domain of PIST was then purified to homogeneity and crystallized at 293 K. Finally, X-ray diffraction data were collected to a resolution of 4.0 angstrom from a crystal belonging to the hexagonal space group P6(2)22 or P6(4)22, with unit-cell parameters a = b = 85.19, c = 240.09 angstrom, gamma = 120.00 degrees
URI
http://hdl.handle.net/YU.REPOSITORY/26085http://dx.doi.org/10.1107/S1744309113007082
ISSN
1744-3091
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이과대학 > 화학생화학부 > Articles
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