Effects of surface camouflaged islet transplantation on pathophysiological progression in a db/db type 2 diabetic mouse model

Title
Effects of surface camouflaged islet transplantation on pathophysiological progression in a db/db type 2 diabetic mouse model
Author(s)
정지헌육심명[육심명]이해신[이해신]안철희[안철희]이동윤[이동윤]변영로[변영로]
Keywords
ZEALAND OBESE MICE; PANCREATIC-ISLETS; INSULIN-RESISTANCE; ALANINE AMINOTRANSFERASE; BLOOD-GLUCOSE; WEIGHT-GAIN; MELLITUS; LANGERHANS; THERAPY; FAILURE
Issue Date
201304
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.433, no.4, pp.513 - 518
Abstract
To investigate the inhibition effects of pancreatic islet transplantation on the progression of obese type 2 diabetes, we analyzed the effects of surface camouflaged islet transplantation on delaying the disease progression in a db/db diabetic mouse model. Surface camouflaged islets using 6-arm-PEG-catechol were transplanted in db/db diabetic mice. The fat accumulation and toxicity in the liver, the expansion of islets in the pancreas, and the size change of abdominal adipocyte were analyzed. In addition, the blood glucose control, insulin levels and immunohistochemical staining of recovered tissues were analyzed after transplantation. Then co-administration of anti-CD154 monoclonal antibody and Tacrolimus (IT group) deterred the pathophysiological progression of obese type 2 diabetes. At day 3 of transplantation, the serum insulin concentration of IT group was increased compared to the db/db diabetic mice group. The immunohistochemical studies demonstrated that the mass of 6-arm-PEG-catechol grafted islet was preserved in the transplantation site for 14 days. Surface modification using 6-arm-PEG-catechol effectively inhibited the immune cell infiltration and activation of host immune cells when immunosuppressive drug was given to the db/db type 2 diabetes mice. Therefore, 6-arm-PEG-catechol grafted islets effectively restored the insulin secretion in islet recipients and prevented the disease progression in type 2 diabetes. (C) 2013 Elsevier Inc. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/26048http://dx.doi.org/10.1016/j.bbrc.2013.03.015
ISSN
0006-291X
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약학대학 > 약학부 > Articles
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