Indole and 7-benzyloxyindole attenuate the virulence of Staphylococcus aureus

Title
Indole and 7-benzyloxyindole attenuate the virulence of Staphylococcus aureus
Author(s)
이진태조무환김정애이진형조현섭수리드반스코타김영훈[김영훈]
Keywords
PSEUDOMONAS-AERUGINOSA VIRULENCE; CAENORHABDITIS-ELEGANS; ESCHERICHIA-COLI; BIOFILM FORMATION; IN-VITRO; STAPHYLOXANTHIN; SIGNAL; RESISTANCE; 7-HYDROXYINDOLE; BIOSYNTHESIS
Issue Date
201305
Publisher
SPRINGER
Citation
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.97, no.10, pp.4543 - 4552
Abstract
Human pathogens can readily develop drug resistance due to the long-term use of antibiotics that mostly inhibit bacterial growth. Unlike antibiotics, antivirulence compounds diminish bacterial virulence without affecting cell viability and thus, may not lead to drug resistance. Staphylococcus aureus is a major agent of nosocomial infections and produces diverse virulence factors, such as the yellow carotenoid staphyloxanthin, which promotes resistance to reactive oxygen species (ROS) and the host immune system. To identify novel antivirulence compounds, bacterial signal indole present in animal gut and diverse indole derivatives were investigated with respect to reducing staphyloxanthin production and the hemolytic activity of S. aureus. Treatment with indole or its derivative 7-benzyloxyindole (7BOI) caused S. aureus to become colorless and inhibited its hemolytic ability without affecting bacterial growth. As a result, S. aureus was more easily killed by hydrogen peroxide (H2O2) and by human whole blood in the presence of indole or 7BOI. In addition, 7BOI attenuated S. aureus virulence in an in vivo model of nematode Caenorhabditis elegans, which is readily infected and killed by S. aureus. Transcriptional analyses showed that both indole and 7BOI repressed the expressions of several virulence genes such as alpha-hemolysin gene hla, enterotoxin seb, and the protease genes splA and sspA and modulated the expressions of the important regulatory genes agrA and sarA. These findings show that indole derivatives are potential candidates for use in antivirulence strategies against persistent S. aureus infection.
URI
http://hdl.handle.net/YU.REPOSITORY/25942http://dx.doi.org/10.1007/s00253-012-4674-z
ISSN
0175-7598
Appears in Collections:
공과대학 > 화학공학부 > Articles
약학대학 > 약학부 > Articles
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