A comparative study on the bulk adhesive strength of the recombinant mussel adhesive protein fp-3

Title
A comparative study on the bulk adhesive strength of the recombinant mussel adhesive protein fp-3
Author(s)
서정현양병선[양병선]강동균[강동균]최유성[최유성]차형준[차형준]
Keywords
MYTILUS-EDULIS; REDOX; EXPRESSION; DOPA; CALIFORNIANUS; COATINGS; LINKING; PLAQUES; BYSSUS; FILMS
Issue Date
201305
Publisher
TAYLOR & FRANCIS LTD
Citation
BIOFOULING, v.29, no.5, pp.483 - 490
Abstract
Mussel adhesive protein (MAP) type 3 (fp-3) is considered one of the key components for mussel adhesion. However, its bulk adhesive strength has not been characterized due to its availability in limited quantities. In the present work, a feasible production (approximate to 47mgl(-1)) of recombinant fp-3 was achieved, and its bulk adhesive strength was measured for the first time; approximate to 0.57MPa for the unmodified form and approximate to 0.94 and approximate to 2.28MPa for the 3,4-dihydroxy-L-phenylalanine (DOPA)-modified form, having a 9.6% yield without and with oxidant treatment, respectively. Furthermore, values for the bulk adhesive strength of several DOPA-modified recombinant MAPs were compared. The maximum adhesive strength of DOPA-modified fp-3 after oxidant treatment was stronger than that of type 5 (fp-5), which has a 6.2% modification yield, and was comparable to that of hybrid types fp-131 and fp-151, which have similar yields (approximate to 5%). The strong bulk adhesive property of recombinant fp-3 demonstrates its potential use as a promising bioadhesive.
URI
http://hdl.handle.net/YU.REPOSITORY/25910http://dx.doi.org/10.1080/08927014.2013.782541
ISSN
0892-7014
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공과대학 > 화학공학부 > Articles
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