Low-Voltage Pulsed Electric Field Sterilization on a Microfluidic Chip

Title
Low-Voltage Pulsed Electric Field Sterilization on a Microfluidic Chip
Author(s)
주상우Linlin Liu[Linlin Liu]Liping Zhao[Liping Zhao]Xiaoping Wan[Xiaoping Wan]닝후Li-Hsien Yeh[Li-Hsien Yeh]Jun Yang[Jun Yang]스즈첸
Keywords
THROUGHPUT CELL ELECTROFUSION; ESCHERICHIA-COLI; MICROORGANISMS; INACTIVATION; BACTERIA; WATER; MICROREACTOR; TEMPERATURE; MEMBRANES; FOODS
Issue Date
201305
Publisher
WILEY-V C H VERLAG GMBH
Citation
ELECTROANALYSIS, v.25, no.5, pp.1301 - 1309
Abstract
A polyimide substrate based microfluidic chip with thousands of comb-shaped microelectrodes has been designed, fabricated, and tested for sterilization of bacteria by using pulsed electric field. The performance of bacteria sterilization as functions of the electric field strength, pulse number and width, treatment buffer, bacteria growth status, and bacteria enrichment by positive dielectrophoresis has been experimentally investigated on the microfluidic chip. Experimental results show that only 100V are sufficient to obtain good sterilization of Escherichia coli. Higher electric field strength, bacteria enrichment by positive dielectrophoresis, longer pulse time, buffer with fewer components and nutritions, and suitable bacteria growth status also improve the sterilization of bacteria. In addition, configuration of the microelectrode array affects bacteria sterilization. This microfluidic device allows one to preconcentrate bacteria to a region with high electric field strength by using positive dielectrophoresis, and subsequently kill the enriched bacteria by applying a pulsed electric field through the same microelectrode array.
URI
http://hdl.handle.net/YU.REPOSITORY/25908http://dx.doi.org/10.1002/elan.201200648
ISSN
1040-0397
Appears in Collections:
공과대학 > 기계공학부 > Articles
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