An ethanol extract of Iris nertschinskia induces p53-dependent apoptosis in the MCF7 human breast cancer cell line

Title
An ethanol extract of Iris nertschinskia induces p53-dependent apoptosis in the MCF7 human breast cancer cell line
Author(s)
홍남주신재식[신재식]홍성우[홍성우]이진견[이진견]이영민[이영민]김대원[김대원]김지은[김지은]정다정[정다정]안성권[안성권]김대진[김대진]진동훈[진동훈]이석용[이석용]
Keywords
CYTOCHROME-C RELEASE; WILD-TYPE P53; BCL-XL; BAX; MITOCHONDRIA; PATHWAY; DEATH; INDUCTION; THERAPY; CASCADE
Issue Date
201101
Publisher
SPANDIDOS PUBL LTD
Citation
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, v.27, no.3, pp.401 - 405
Abstract
Iris nertschinskia, an ornamental plant, is utilized in traditional East Asian medicine for the treatment of skin diseases. However, the biological activity underlying its therapeutic effects remains to be established. In this study, we investigated the anti-tumor effect of the plant extract on MCF7 human breast cancer cells. An ethanol extract of Iris nertschinskia triggered cell death in a dose-dependent manner. Moreover, treatment with the extract promoted p53 phosphorylation in MCF7 cells. Increased phosphorylation of p53, in turn, led to induction of Bax protein, a key regulator of p53-dependent apoptotic cell death, as well as of caspase-7 cleavage in MCF7 cells. Consistently, cells treated with p53-specific siRNA or the caspase inhibitor, Z-VAD, resisted apoptotic cell death induced by the Iris nertschinskia extract. Our results suggest that p53 sensitizes tumor cells to the ethanol extract of Iris nertschinskia by Bax protein induction and caspase-dependent apoptosis.
URI
http://hdl.handle.net/YU.REPOSITORY/25786http://dx.doi.org/10.3892/ijmm.2011.599
ISSN
1107-3756
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생명공학부 > 생명공학부 > Articles
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