Anti-inflammatory action of methanol extract of Carthamus tinctorius involves in heme oxygenase-1 induction

Title
Anti-inflammatory action of methanol extract of Carthamus tinctorius involves in heme oxygenase-1 induction
Author(s)
이승호장기철[장기철]전민수[전민수]하유미[하유미]김희숙[김희숙]장화진[장화진]김영민[김영민]이영수[이영수]김혜정[김혜정]서한극[서한극]이재흔[이재흔]
Keywords
CELL-ADHESION MOLECULE-1; SMOOTH-MUSCLE-CELLS; RHEUMATOID-ARTHRITIS; OXIDATIVE DAMAGE; BINDING-SITE; EXPRESSION; HEME-OXYGENASE-1; INFLAMMATION; ENDOTHELIUM; DEFICIENCY
Issue Date
201101
Publisher
ELSEVIER IRELAND LTD
Citation
JOURNAL OF ETHNOPHARMACOLOGY, v.133, no.2, pp.524 - 530
Abstract
Ethnopharmacological relevance: The methanol extracts of Carthamus tinctorius (MEC) have long been used in traditional medicine as anti-inflammatory agent, however, the molecular mechanism by which MEC shows anti-inflammatory action is not investigated. Aim of the study: Induction of heme oxygenase-1 (HO-1) by many medicinal herbs has been reported excellent anti-inflammatory action. Thus, the aim of the study is to explore whether anti-inflammatory action of MEC is related with HO-1 induction in RAW 264.7 cells. Materials and methods: The present study was designed to investigate as to MEC induces HO-1 expression so that it reduces inflammation by suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression in cells activated with lipopolysaccharide (LPS). Results: Expression of HO-1 protein by MEC in macrophages was increased in a concentration- and time-dependent manner. Treatment with MEC significantly inhibited upregulation of both iNOS and COX-2 in LPS-activated macrophages and consequently reduced production of NO and PGE(2), respectively. The reduced expression of iNOS and COX-2 by MEC was reversed by siHO-1 RNA transfection. In addition, NF-E2-related factor (Nrf2) was translocated from cytosol to nucleus by MEC. The binding of NF-kappa B as well as NF-kappa B luciferase activity was also significantly diminished by MEC. Finally, tumor necrosis factor (TNF)-alpha-mediated VCAM-1 expression in endothelial cell was significantly inhibited by MEC. Conclusions: The present results show that MEC induces HO-1 expression via Nrf2 translocation and inhibits NF-kappa B activity, which may be responsible for anti-inflammatory action. Therefore, we propose that anti-inflammatory action of MEC involves at least HO-1 induction. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/25757http://dx.doi.org/10.1016/j.jep.2010.10.029
ISSN
0378-8741
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약학대학 > 약학부 > Articles
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