Determination of a novel low-voltage-activated calcium channel blocker (HYP-10) in rat plasma by liquid chromatography-mass spectrometry

Title
Determination of a novel low-voltage-activated calcium channel blocker (HYP-10) in rat plasma by liquid chromatography-mass spectrometry
Author(s)
노금한[노금한]김서영[김서영]감유림[감유림]박혜영[박혜영]이화정[이화정]강원구
Issue Date
201102
Publisher
ELSEVIER SCIENCE BV
Citation
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v.54, no.3, pp.568 - 571
Abstract
A novel T-type calcium channel blocker, 4-amino-1-{4-[(4-chloro-phenyl)-Phenyl-methyl]-piperazin-1-yl}-butan-1-one (HYP-10) has been synthesized, and the compound has shown promise as both a nociceptive and inflammatory pain reliever as well as an analgesic in a rat neuropathic pain model. A quantification method was developed for the determination of HYP-10 in rat plasma. After simple protein precipitation with methanol, HYP-10 and the internal standard, methaqualone were chromatographed on a reversed-phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma HYP-10 concentration after a single intravenous administration of the compound in rats. (C) 2010 Elsevier B.V. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/25661http://dx.doi.org/10.1016/j.jpba.2010.10.007
ISSN
0731-7085
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약학대학 > 약학부 > Articles
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