Simultaneous Determination of Bioactive Flavonoids in Some Selected Korean Thistles by High-Performance liquid Chromatography

Title
Simultaneous Determination of Bioactive Flavonoids in Some Selected Korean Thistles by High-Performance liquid Chromatography
Author(s)
손종근Nguyen Thi Phuong Th[Nguyen Thi Phuong Th]To Dao Cuong1[To Dao Cuong1]Tran Manh Hung1[Tran Manh Hung1]이제현[이제현]나민균정현주[정현주]방철[방철]우미희[우미희]최재수[최재수]민병선[민병선]
Keywords
CIRSIUM-JAPONICUM DC; LIPID-PEROXIDATION; MILK THISTLE; SILYMARIN; HEPATOTOXICITY; INHIBITION; USSURIENSE; PRINCIPLE; MICE
Issue Date
201103
Publisher
PHARMACEUTICAL SOC KOREA
Citation
ARCHIVES OF PHARMACAL RESEARCH, v.34, no.3, pp.455 - 461
Abstract
In order to facilitate the quality control of some selected Korean thistles (Cirsii Herb), Cirsium japonicum var ussuriense, C. japonium var spinosissimum, C. setidens, C. pendulum, C. nipponicum, Carduus crispus, and Breea segetum, a simple, accurate and reliable high performance liguid chromatography method was developed for the simultaneous determination of the six flavonoids: luteolin 5-O-glucoside (1), luteolin 7-O-glucoside (2), hispidulin 7-O-neohesperidoside (3), luteolin (4), pectolinarin (5), and apigenin (6), which were selected as the chemical markers of the thistles. Separation was achieved on an Agilent Eclipse XDB-C18 column with a gradient solvent system of 0.1% trifluoroacetic acid aqueous-methanol at a flow-rate of 1.0 mL/min and detected at 254 nm. All six calibration curves showed good linearity (R(2) > 0.991). The method was reproducible with intra-and inter-day variations of less than 6%. The recoveries were in the range of 90.01-100.05%. This analysis method was successfully utilized to quantify the six flavonoids in the 22 batches of the thistles. The results demonstrated that this method is simple, reliable and suitable for the quality control of this medicinal herb.
URI
http://hdl.handle.net/YU.REPOSITORY/25523http://dx.doi.org/10.1007/s12272-011-0314-x
ISSN
0253-6269
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약학대학 > 약학부 > Articles
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