Mechanism of DNA Cleavage Induced by Fe2+ Autoxidation

Title
Mechanism of DNA Cleavage Induced by Fe2+ Autoxidation
Author(s)
김종문김석규
Keywords
SINGLET MOLECULAR-OXYGEN; LABILE IRON POOL; HYDROGEN-PEROXIDE; STRAND SCISSION; FENTON REACTION; LIPID-PEROXIDATION; RADICAL FORMATION; OXIDATIVE STRESS; XANTHINE-OXIDASE; FERROUS IRON
Issue Date
201103
Publisher
KOREAN CHEMICAL SOC
Citation
BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.32, no.3, pp.964 - 972
Abstract
This work investigated the difference between Fe2+ autoxidation-induced and Fenton-type cleavage of pBR322 plasmid DNA. (OH)-O-center dot generation reactions in the absence and presence of H2O2 under various conditions were also investigated. Although both the Fe2+ autoxidation and Fenton-type reactions showed DNA cleavage and (OH)-O-center dot generation, there were significant differences in their efficiencies and reaction rates. The rate and efficiency of the cleavage reaction were higher in the absence of 1.0 mM of H2O2 than in its presence in 20 mM phosphate buffer. In contrast, the (OH)-O-center dot generation reaction was more prominent in the presence of H2O2 and showed a pH-independent, fast initial reaction rate, but the rate was decreased in the absence of H2O2 at across the entire tested pH range. Studies using radical scavengers on DNA cleavage and (OH)-O-center dot generation reactions in both the absence and presence of H2O2 confirmed that both reactions spontaneously involved the active oxygen species (OH)-O-center dot, O-2(center dot-), O-1(2) and H2O2, indicating that a similar process may participate in both reactions. Based on the above observations, a new mechanism for the Fe2+ autoxidation-induced DNA cleavage reaction is proposed.
URI
http://hdl.handle.net/YU.REPOSITORY/25516http://dx.doi.org/10.5012/bkcs.2011.32.3.964
ISSN
0253-2964
Appears in Collections:
문과대학 > 중국언어문화학과 > Articles
이과대학 > 화학생화학부 > Articles
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