Generalized semi-refolding methods for purification of the functional death domain superfamily

Title
Generalized semi-refolding methods for purification of the functional death domain superfamily
Author(s)
박현호장태호[장태호]
Keywords
AIM2 INFLAMMASOME; CRYSTAL-STRUCTURE; NMR STRUCTURE; PYRIN DOMAIN; ACTIVATION; RAIDD; PIDDOSOME; MECHANISM; APOPTOSIS; PROTEINS
Issue Date
201103
Publisher
ELSEVIER SCIENCE BV
Citation
JOURNAL OF BIOTECHNOLOGY, v.151, no.4, pp.335 - 342
Abstract
The death domain (DD) superfamily comprising the death domain (DD) subfamily, the death effector domain (DED) subfamily, the caspase recruitment domain (CARD) subfamily and the pyrin domains (PYD) subfamily is one of the largest classes of protein interaction modules and plays a pivotal role in the apoptosis, inflammation, and immune cell signaling pathways. Despite the biological importance of the death domain superfamily, structural and in vitro biochemical studies have been limited because these domains are prone to aggregate under physiological conditions. Here, we describe a generalized method, termed semi-refolding. that is particularly applicable for purification of the functional death domain superfamily. The recombinant proteins Caspase-1 CARD, AIM2 PYD, NALP3 PYD, and RIM DD from inclusion bodies were successfully purified using this method. (C) 2011 Elsevier B.V. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/25502http://dx.doi.org/10.1016/j.jbiotec.2011.01.003
ISSN
0168-1656
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이과대학 > 화학생화학부 > Articles
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