누에(Bombyx mori)로부터 분리한 렉틴의 생화학적 특성

Title
누에(Bombyx mori)로부터 분리한 렉틴의 생화학적 특성
Other Titles
Biochemical Properties of the Lectin Isolated from Bombyx mori
Author(s)
전경희김세진[김세진]이상용[이상용]
Keywords
Bombyx mori; Lectins; Hemagglutination
Issue Date
201103
Publisher
한국생약학회
Citation
생약학회지, v.42, no.1, pp.68 - 75
Abstract
A new lectin was purified from Bombyx mori (BML) by physiological saline extraction, ammonium sulfate precipitants,anion exchange column chromatography on DEAE Sephadex A-50 and gel filtration column chromatography on Sephadex G-200. BML agglutinated trypsinized and glutaraldehyde-fixed erythrocytes, and was observed the most high activity with rabbit, chicken erythrocytes and rat splenic lymphocytes. Agglutinability was markedly affected at highly acidic pH, but was relatively stable with high temperature. The effect of metal ions was observed and BML was affected by bivalaent cations,especially depending on Ca^(2+), Fe^(2+), Mn^(2+), whereas, inhibited by Mg^(2+). Agglutination was strongly inhibited by heparin and glucuronic acid. BML was proved to be a glycoprotein which contains 17.16% of sugars. By mass spectrometry analysis, we found 2 bands that were considered as lectin subunits.
URI
http://hdl.handle.net/YU.REPOSITORY/25418
ISSN
0253-3073
Appears in Collections:
이과대학 > 생명과학과 > Articles
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