E6 and E7 fusion immunoglobulin from human papilloma virus 16 induces dendritic cell maturation and antigen specific activation of T helper 1 response

Title
E6 and E7 fusion immunoglobulin from human papilloma virus 16 induces dendritic cell maturation and antigen specific activation of T helper 1 response
Author(s)
최인호김상훈[김상훈]허유진[허유진]이석준[이석준]김상준[김상준]박정규[박정규]오유경[오유경]정운원[정운원]서정복[서정복]남명희[남명희]전태훈[전태훈]
Keywords
PROTEIN; VACCINE; INFECTION; IMMUNITY; IMMUNIZATION; INDUCTION; NEOPLASIA; CANCERS; SAFETY
Issue Date
201104
Publisher
SPRINGER
Citation
BIOTECHNOLOGY LETTERS, v.33, no.4, pp.663 - 671
Abstract
Human papilloma virus (HPV) 16 causes cervical cancer. Induction of oncogenesis by HPV 16 is primarily dependent on the function of E6 and E7 proteins, which inactivate the function of p53 and pRB, respectively. Thus, blocking the activity of the E6 and E7 proteins from HPV 16 is critical to inhibiting oncogenesis during infection. We have expressed and purified soluble HPV 16 E6 and E7 fusion immunoglobulin (Ig), which were combined with the constant region of an Ig heavy chain, in a mammalian system. To assess whether soluble E6 and E7 fusion Igs induce effective cellular immune responses, immature dendritic cells (DCs) were treated with these fusion proteins. Soluble E6 and E7 fusion Igs effectively induced maturation of DCs. Furthermore, immunization with soluble E6 and E7 fusion Igs in mice resulted in antigen-specific activation of T helper 1 (Th1) cells. This is the first comprehensive study to show the molecular basis of how soluble HPV 16 E6 or E7 fusion Igs induces Th1 responses through the maturation of DCs. In addition, we show that DC therapy using soluble HPV E6 and E7 fusion Igs may be a valuable tool for controlling the progress of cervical cancer.
URI
http://hdl.handle.net/YU.REPOSITORY/25406http://dx.doi.org/10.1007/s10529-010-0489-0
ISSN
0141-5492
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