Underestimated biodiversity as a major explanation for the perceived rich secondary metabolite capacity of the cyanobacterial genus Lyngbya

Title
Underestimated biodiversity as a major explanation for the perceived rich secondary metabolite capacity of the cyanobacterial genus Lyngbya
Author(s)
최혁재Neclas Engene[Neclas Engene]Eduardo Esquenazi[Eduardo Esquenazi]Erin C. Rottacker[Erin C. Rottacker]Mark H. Ellisman[Mark H. Ellisman]Pieter C. Dorrestein[Pieter C. Dorrestein]Willam H. Gerwick[Willam H. Gerwick]
Keywords
MARINE CYANOBACTERIUM; NATURAL-PRODUCTS; CURACIN-A; MAJUSCULA; CLUSTER; OSCILLATORIALES; DIVERSITY; BARBAMIDE; ORIGIN
Issue Date
201104
Publisher
WILEY-BLACKWELL
Citation
ENVIRONMENTAL MICROBIOLOGY, v.13, no.6, pp.1601 - 1610
Abstract
Marine cyanobacteria are prolific producers of bioactive secondary metabolites responsible for harmful algal blooms as well as rich sources of promising biomedical lead compounds. The current study focused on obtaining a clearer understanding of the remarkable chemical richness of the cyanobacterial genus Lyngbya. Specimens of Lyngbya from various environmental habitats around Curacao were analysed for their capacity to produce secondary metabolites by genetic screening of their biosynthetic pathways. The presence of biosynthetic pathways was compared with the production of corresponding metabolites by LC-ESI-MS(2) and MALDI-TOF-MS. The comparison of biosynthetic capacity and actual metabolite production revealed no evidence of genetic silencing in response to environmental conditions. On a cellular level, the metabolic origin of the detected metabolites was pinpointed to the cyanobacteria, rather than the sheath-associated heterotrophic bacteria, by MALDI-TOF-MS and multiple displacement amplification of single cells. Finally, the traditional morphology-based taxonomic identifications of these Lyngbya populations were combined with their phylogenetic relationships. As a result, polyphyly of morphologically similar cyanobacteria was identified as the major explanation for the perceived chemical richness of the genus Lyngbya, a result which further underscores the need to revise the taxonomy of this group of biomedically important cyanobacteria.
URI
http://hdl.handle.net/YU.REPOSITORY/25398http://dx.doi.org/10.1111/j.1462-2920.2011.02472.x
ISSN
1462-2912
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약학대학 > 약학부 > Articles
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