Reverse migration order of sibutramine enantiomers as a function of cyclodextrin concentration in capillary electrophoresis

Title
Reverse migration order of sibutramine enantiomers as a function of cyclodextrin concentration in capillary electrophoresis
Author(s)
Hongmei Zhu[Hongmei Zhu]Enqi Wu[Enqi Wu]Jianbo CHen[Jianbo CHen]장유선[장유선]강원구최정갑[최정갑]이원재[이원재]강종성[강종성]
Keywords
AMINO-ACID-SEQUENCE; BETA-CYCLODEXTRIN; ZONE-ELECTROPHORESIS; BINDING CONSTANTS; CHIRAL SELECTOR; DERIVATIZED CYCLODEXTRINS; TRIPEPTIDE ENANTIOMERS; SEPARATION; ENANTIOSEPARATION; PH
Issue Date
201104
Publisher
ELSEVIER SCIENCE BV
Citation
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v.54, no.5, pp.1007 - 1012
Abstract
The current study demonstrates the reversal of enantiomer migration order (EMO) in capillary electrophoresis (CE) based separations of sibutramines (SIB) as a function of the concentration of two types of cyclodextrin (CD), native beta-CD and acetyl-beta-CD. At normal working concentrations (<10 mM) of either CD, (S)-SIB migrated first. However, at CD concentrations greater than 10 mM, (R)-SIB was the first to migrate. This study describes factors involved in determining EMO for sibutramine enantiomers at low and high concentrations of CDs. The reversal of EMO could be explained in terms of the opposing effects of the stability and the limiting complex mobility of the SIB-CD complexes. The enantioseparation of SIB with methyl- and 2-hydroxypropyl-beta-CD was possible based on differences in the binding constants of complexes. However, reverse EMO was not observed because of equal mobilities of SIB enantiomers complexed with methyl- and 2-hydroxypropyl-beta-CD. (C) 2010 Elsevier B.V. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/25373http://dx.doi.org/10.1016/j.jpba.2010.12.001
ISSN
0731-7085
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약학대학 > 약학부 > Articles
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