Chemical Constituents from the Sclerotia of Inonotus obliquus

Title
Chemical Constituents from the Sclerotia of Inonotus obliquus
Author(s)
심상희김유진[김유진]박진선[박진선]민병선[민병선]
Keywords
PERSOON PILAT; CELL-LINE; IN-VIVO; CHAGA; CYTOTOXICITY; INOTODIOL; EXTRACT; TRITERPENE; APOPTOSIS
Issue Date
201104
Publisher
KOREAN SOC APPLIED BIOLOGICAL CHEMISTRY
Citation
JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY, v.54, no.2, pp.287 - 294
Abstract
Six triterpenoids, two sterols, and two phenolic constituents were isolated from the methanol extract of sclerotia of Inonotus obliquus (Hymenochaetaceae). Their chemical structures were identified as lanosterol (1), 3 beta-hydroxylanosta-8,24-dien-21-al (3), inotodiol (5), trametenolic acid (6), lanosta-8,25-dien-3,22,24-triol (7), beta-sitosterol (2), ergosterolperoxide (4), betulin (8), 2,5-dihydroxy-benzaldehyde (9), and 3,4-dihydroxybenzalacetone (10) on the basis of spectroscopic methods and comparison with the literature. Among these compounds, 9 was isolated for the first time from this mushroom. To the best of our knowledge, the present study marks the first chemical investigation of I. obliquus from Alaska. Among the isolated compounds, compounds 1, 3, 4, 5, 6, 8, 9, and 10 were evaluated for in vitro cytotoxic activity against A 549 (human alveolar basal epithelial carcinoma cells), L1210 (mouse lymphocytic leukemia cells), COLO 205 (colorectal adenocarcinoma cells), MCF-7 (breast cancer cells), and HL-60 (human leukemia) cancer cell lines. 3 beta-Hydroxylanosta-8,24-dien-21-al (3) and tramentenolic acid (6) exhibited modest cytotoxic effects against L1210 cells with IC(50)s of 62.5 and 34.4 mu M, and betulin (8) showed weak cytotoxic effects against A549 and HL-60 with IC(50)s of 81.2 and 87.5 mu M, respectively. In addition, 3,4-dihydroxybenzalacetone (10) exhibited the strongest cytotoxic activities against A549 and HL-60 cells with IC(50) values of 23.6 and 21.7 mu M, respectively.
URI
http://hdl.handle.net/YU.REPOSITORY/25364http://dx.doi.org/10.3839/jksabc.2011.045
ISSN
1738-2203
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생명공학부 > 생명공학부 > Articles
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