A method to determine quasi-steady state in constant voltage mode isotachophoresis

Title
A method to determine quasi-steady state in constant voltage mode isotachophoresis
Author(s)
심재술Prashanta Dutta[Prashanta Dutta]조미경[조미경]
Keywords
SEPARATION CHANNELS; PLANAR CHIP; DISPLACEMENT ELECTROPHORESIS; TRANSIENT ISOTACHOPHORESIS; CAPILLARY-ELECTROPHORESIS; CONDUCTIVITY DETECTION; ZONE ELECTROPHORESIS; COMPUTER-SIMULATION; PROTEIN; COLUMN
Issue Date
201104
Publisher
WILEY-BLACKWELL
Citation
ELECTROPHORESIS, v.32, no.9, pp.988 - 995
Abstract
Identification of the steady state is very challenging in isotachophoresis (ITP); especially in complex microgeometries, such as dog-leg channels or cross-channel junctions. In this work, an elastic matching method is applied to determine the quasi-steady state in microscale ITP. In the elastic matching method, the similarity between two profiles is calculated by comparing intensity distribution of two images or profiles. To demonstrate this similarity-based analysis technique for ITP, a constant voltage mode ITP model is developed and applied to a five-component ITP system. Hydrochloric acid and caproic acid are used as the leader and terminator, respectively, while histidine is used as the counterion. Two sample components, acetic acid and benzoic acid, are separated under the action of an applied electric field in both straight and dog-leg microchannels. This analysis shows that conductivity profiles provide a better measure to determine the quasi-steady state in an ITP process. For a straight microchannel, the quasi-steady state is achieved in less than a minute with a total potential drop of 100 V in a 2 cm long channel. In a straight channel, a true steady state can be achieved for ITP with appropriate countercurrent flow where stationary zones are formed, but the time it takes to reach the steady state is much longer than the without counter flow case. The numerical results indicate that a steady state cannot be reached in a dog-leg microchannel because of sample dispersion and refocusing at and near the intersections and at the branch channels. However, the elastic matching method can be used to determine the quasi-steady state in a dog-leg microchannel.
URI
http://hdl.handle.net/YU.REPOSITORY/25353http://dx.doi.org/10.1002/elps.201000594
ISSN
0173-0835
Appears in Collections:
공과대학 > 기계공학부 > Articles
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