Chloroform extract of garlic (Allium sativum) stimulates production of superoxide anion and CXCL8 in human neutrophils

Title
Chloroform extract of garlic (Allium sativum) stimulates production of superoxide anion and CXCL8 in human neutrophils
Author(s)
백석환심재웅[심재웅]이하영[이하영]김상두[김상두]김학정[김학정]박준성[박준성]김선근[김선근]최영환[최영환]배외식[배외식]
Keywords
HUMAN MONOCYTES; PEPTIDE; IDENTIFICATION; GENERATION; LEUKOCYTES; RECEPTORS; MEDICINE
Issue Date
201104
Publisher
KOREAN SOC HORTICULTURAL SCIENCE
Citation
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY, v.52, no.2, pp.218 - 223
Abstract
Garlic has garnered attention as a useful material which contains many bioactive molecules for the prevention and/or treatment of various human ailments, including infectious diseases. In this study we tested several extracts of garlic on cellular activity in human neutrophils. Stimulation of human neutrophils with chloroform extract of garlic (GCE) elicited intracellular calcium ([Ca(2+)](i)) increases in a phospholipase C independent manner. GCE also stimulated superoxide anion production, which was completely blocked by a calcium (Ca(2+)) chelator. We also observed that stimulation of human neutrophils with GCE caused production of the chemokine CXCL8 in a concentration-dependent manner. GCE also stimulated activity of two important mitogen activated protein kinases (MAPKs), extracellular signal-regulated kinase (ERK) and p38 MAPK. Moreover, GCE-induced CXCL8 production was inhibited by a p38 MAPK inhibitor (SB203580) but not by an ERK inhibitor (PD98059). Taken together, our results demonstrate that GCE contains bioactive natural compounds that stimulate [Ca(2+)](i) signaling, superoxide anion production, and CXCL8 production. Thus, GCE may be useful for the development of immune-modulating agents against infectious diseases.
URI
http://hdl.handle.net/YU.REPOSITORY/25350http://dx.doi.org/10.1007/s13580-011-0212-7
ISSN
2211-3452
Appears in Collections:
의과대학 > 생화학.분자생물학교실 > Articles
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