RAPD와 SRAP 방법을 이용한 ‘성전온주’(C. unshiu Marc.)와 ‘병감’(C. reticulate Blanco) 교잡실생 식별

Title
RAPD와 SRAP 방법을 이용한 ‘성전온주’(C. unshiu Marc.)와 ‘병감’(C. reticulate Blanco) 교잡실생 식별
Other Titles
Early Identification of Putative Zygotic Seedlings in Citrus Crosses between ‘Morita unshiu’ (Citrus. unshiu Marc.) and ‘Ponkan’ (C. reticulata Blanco) Using RAPD and SRAP
Author(s)
문용선윤수현[윤수현]진성범[진성범]강인규[강인규]이동훈[이동훈]
Keywords
DNA marker; hybrid zygotic seedling; polyembryony; polymorphic bands
Issue Date
201104
Publisher
한국생명과학회
Citation
생명과학회지, v.21, no.4, pp.502 - 508
Abstract
The purpose of this study was to evaluate the methods of identifying zygotic seedlings of crosses between ‘Morita unshiu’ (Citrus. unshiu Marc.) and ‘Ponkan’ (C. reticulata Blanco). In order to investigate the frequency and position of zygotic seedlings and to determine the efficiency of zygotic seedling identification, random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) were performed using UBC (9, 27, 229, 230, and 254) primers and F4/R27, F7/R14, F12/R10, and F44/R62 primer sets, respectively. A total of 37 putative zygotic seedlings out of 55 individuals were selected by RAPD and SRAP. The F7/R14 primer pair showed a screening ability of 45.5% (25/55), whereas the primer UBC27 revealed the highest efficiency of zygotic seedling identification (50.9%, 28/55). When both UBC27 and F7/R14 were properly used for selection of hybridized seedlings of ‘Morita unshiu’ (C. unshiu Marc.) and ‘Ponkan’ (C. reticulata Blanco), screening efficiency was increased to 60% (33/55) for putative zygotic seedlings. Thus, it is possible to select putative hybrid zygotic seedlings in an accurate and effective manner by RAPD and SRAP.
URI
http://hdl.handle.net/YU.REPOSITORY/25338
ISSN
1225-9918
Appears in Collections:
자연자원대학 > 원예생명과학과 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE