Growth-Suppressing Activity of the Transfected Cx26 on BICR-M1Rk Breast Cancer Cell Line

Title
Growth-Suppressing Activity of the Transfected Cx26 on BICR-M1Rk Breast Cancer Cell Line
Author(s)
이승근이해정[이해정]
Keywords
C6 GLIOMA-CELLS; GAP-JUNCTION PROTEIN; NUCLEAR EXPORT SIGNAL; CONNEXIN GENES; NEGATIVE REGULATION; POSSIBLE MECHANISM; TRANSFORMED-CELLS; TUMOR-CELLS; PROLIFERATION; EXPRESSION
Issue Date
201105
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.21, no.5, pp.477 - 482
Abstract
There are accumulating evidences suggesting that connexin (Cx), a gap junction channel-forming protein, acts as a growth suppressor in various cancer cells, and this effect is attributeed to the gap junction-mediated intercellular communication (GJIC). In order to characterize the relationship between the growth-arresting activity of Cx26 and its cytoplasmic localizations after expression, we linked a nuclear export signal (NES) sequence to Cx26 cDNA before transfecting into a rat breast cancer cell line. A confocal fluorescent microscopic observation revealed that the insertion of NES minimized the nuclear expression of Cx26, and increased its cytoplasmic expression, including plasma membrane junctions. Total cell counting and BrdUrd-labeling experiments showed that the growth of the breast cancer cells was inhibited by 74% upon transfection of Cx26-NES, whereas only 9% inhibition was observed with only Cx26 cDNA.
URI
http://hdl.handle.net/YU.REPOSITORY/25270http://dx.doi.org/10.4014/jmb.1012.12035
ISSN
1017-7825
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이과대학 > 화학생화학부 > Articles
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