Suppression of Nrf2 Signaling by Angiotensin II in Murine Renal Epithelial Cells

Title
Suppression of Nrf2 Signaling by Angiotensin II in Murine Renal Epithelial Cells
Author(s)
곽미경강수진Aram You[Aram You]
Keywords
SMOOTH-MUSCLE-CELLS; OXIDATIVE STRESS; MESENCHYMAL TRANSITION; PROTEASOMAL DEGRADATION; MOLECULAR-MECHANISMS; KIDNEY FIBROSIS; GENE-EXPRESSION; TGF-BETA; PATHWAY; RATS
Issue Date
201105
Publisher
PHARMACEUTICAL SOC KOREA
Citation
ARCHIVES OF PHARMACAL RESEARCH, v.34, no.5, pp.829 - 836
Abstract
Angiotensin II (ATII), a physiologically active mediator of the renin-angiotensin system, has been shown to be associated with renal fibrosis pathophysiology. In our current study with murine renal epithelial TCMK-1 cells, ATII inhibited the expression of NF-E2-related factor 2 (Nrf2)-target antioxidant genes such as NAD(P)H: quinone oxidoreductase-1 and gamma-glutamate cysteine ligase (GCL). In accord to the reduced expression of GCL, a GSH biosynthesis enzyme, ATII treatment reduced intracellular GSH content and increased the levels of reactive oxygen species (ROS). While, there was no significant changes in nuclear protein levels of Nrf2 following ATII treatment, implying the potential alterations in the expression of partner proteins of Nrf2. Our measurement of Nrf2 partner bZIP transcription factors, including Bach1, small Maf proteins, and activating transcription factor 3 (ATF3) showed that the expression level for ATF3 was enhanced in ATII-treated TCMK cells. Presumably through ATII-induced oxidative stress, ATII treatment was found to enhance the inducible level of TGF beta-mediated alpha-smooth muscle actin (alpha-SMA) expression, which is one of molecular markers of renal fibrosis. In conclusion, our results indicate that ATII suppresses Nrf2-GSH signaling in murine renal epithelial cells. Elevated ROS levels in ATII exposed epithelial cells might be a causing factor contributing to renal fibrosis pathology.
URI
http://hdl.handle.net/YU.REPOSITORY/25258http://dx.doi.org/10.1007/s12272-011-0517-1
ISSN
0253-6269
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약학대학 > 약학부 > Articles
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