Kinetic Resolution of 3-Fluoroalanine Using a Fusion Protein of D-Amino Acid Oxidase with Vitroscilla Hemoglobin

Title
Kinetic Resolution of 3-Fluoroalanine Using a Fusion Protein of D-Amino Acid Oxidase with Vitroscilla Hemoglobin
Author(s)
윤형돈서영만[서영만]강용호
Keywords
VIBRIO-FLUVIALIS JS17; TRANSAMINASE; ALANINE
Issue Date
201105
Publisher
TAYLOR & FRANCIS LTD
Citation
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.75, no.4, pp.820 - 822
Abstract
In this study, a fusion protein (VHb-DAAO) of D-amino acid oxidase (DAAO) with Vitreoscilla hemoglobin (VHb) was functionally expressed in Escherichia colt and purified. The k(cat) value VHb-DAAO (47.1s(-1)) towards rac-3-flouroalanine was about 2-fold higher than that of DAAO (21.9s(-1)). rac-3-Flouroalanine (500 mm) was kinetically resolved into (R)-3-fluoroalanine with high enatiomeric excess (> 99%) by VHb-DAAO with about 52% conversion.
URI
http://hdl.handle.net/YU.REPOSITORY/25248http://dx.doi.org/10.1271/bbb.110122
ISSN
0916-8451
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생명공학부 > 생명공학부 > Articles
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