Enhanced Antioxident Effect by over Expression of Tomato beta-carotene Hydroxylase Gene (ChyB) Using Agrobacterium-infiltration in Tobacco Plant
- Enhanced Antioxident Effect by over Expression of Tomato beta-carotene Hydroxylase Gene (ChyB) Using Agrobacterium-infiltration in Tobacco Plant
- 문용선; 최윤정[최윤정]; 윤경영; 윤해근; 서상곤
- PIGMENT BIOSYNTHESIS; LYCOPENE; FRUIT; CYCLASE; CLONING; MAIZE
- Issue Date
- KOREAN SOC HORTICULTURAL SCIENCE
- KOREAN JOURNAL OF HORTICULTURAL SCIENCE & TECHNOLOGY, v.29, no.3, pp.267 - 272
- Several reports indicated that astaxanthin and zeaxanthin have more active anticancer activity than pro-vitamin A carotenes. beta-carotene hydroxylase is a key enzyme to synthesize zeaxanthin and astaxanthin in carotenoids biosynthesis pathway. We isolated the ChyB gene encoding beta-carotene hydroxylase from tomato leaves. The ChyB gene (1.5Kbp) fragment was cloned into the binary vector and designated to pIG121-ChyB-tom. Agrobacterium-mediated infiltration was used for transient assay in Nicotiana benthamiana. Leaf samples were collected 0, 1, 2, 3 days after infiltration (DAD. RT-PCR result showed that the expression of beta-carotene hydroxylase transcripts was not detected in control (0DAI), but its expression was detected after 1 DPI and increased later on. When the activity of beta-carotene hydroxylase was measured, the 1,1-diphenyl-priciyl hydrazyl (DPPH) radical scavenging activity (27%) at 2 DAI was significantly higher than that (21%) at 0 DAI. These results indicated that anti-oxidant activity dramatically increased at 2 DAI in tobacco leaves was due to over expression of tomato beta-carotene hydroxylase. These results can be the foundation to develop tomato cultivars with high oxy-carotenoids content using the ChyB gene transformation.
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