Anti-obesity effect of grape skin extract in 3T3-L1 adipocytes

Title
Anti-obesity effect of grape skin extract in 3T3-L1 adipocytes
Author(s)
조경현정유석[정유석]정희경[정희경]윤광섭[윤광섭]홍주헌[홍주헌]
Keywords
CONJUGATED LINOLEIC-ACID; INHIBITS ADIPOGENESIS; DIFFERENTIATION; APOPTOSIS; RESVERATROL; PROTEIN; PREADIPOCYTES; QUANTITATION; ADIPONECTIN; POLYPHENOLS
Issue Date
201106
Publisher
SPRINGER
Citation
FOOD SCIENCE AND BIOTECHNOLOGY, v.20, no.3, pp.635 - 642
Abstract
The aim of the present study was to determine the effects of grape skin which was well known as natural antioxidant using 3T3-L1 adipocyte, representative cells with morphological and biochemical characteristics of adipocytes. Grape skin extract (GSE) suppressed the differentiation of 3T3-L1 adipocytes, and decreased the triglycerides content with lipid accumulation. The adipogenesis inhibitory effect of GSE was confirmed in adipocyte hormone secretion, such as leptin and adiponectin, as well as glycerol-3-phosphate dehydrogenase activity. However, apoptosis of both mature pre-adipocytes and adipocytes was not induced by GSE. The level of gene and protein expression was observed to understand the antiobesity mechanism underlying GSE. GSE treatment of 3T3-L1 cells resulted in decrease in the adipogenic transcription factor (PPAR gamma, C/EBP alpha, and SREBP1) gene and protein expression, and preadipocyte secreted factor-1 mRNA was up-regulated compared to the adipocyte control. The lipolysis-related mRNA level (hormone-sensitive lipase, lipoprotein lipase, and perilipin) in adipocytes with the addition of grape skin was similar to preadipocytes. These results support the potential application of GSE as a natural anti-obesity material in the food industry.
URI
http://hdl.handle.net/YU.REPOSITORY/25121http://dx.doi.org/10.1007/s10068-011-0090-x
ISSN
1226-7708
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생명공학부 > 생명공학부 > Articles
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