Anti-inflammatory activity of ethylacetate fraction of Cliona celata

Title
Anti-inflammatory activity of ethylacetate fraction of Cliona celata
Author(s)
장현욱양주혜[양주혜]서석종유예[유예]이선[이선]이연경[이연경]장영채[장영채]나민균최정혜[최정혜]김철호[김철호]손종근
Keywords
NF-KAPPA-B; NITRIC-OXIDE SYNTHASE; MATRIX-METALLOPROTEINASE-9 EXPRESSION; GENE-EXPRESSION; INHIBITION; CELLS; MACROPHAGES; ACTIVATION; INDUCTION; GAMMA
Issue Date
201106
Publisher
INFORMA HEALTHCARE
Citation
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY, v.33, no.2, pp.373 - 379
Abstract
We evaluated the ability of the ethylacetate fraction of marine sponge, Cliona celata (ECC), harvested from Korean seaside to regulate the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated murine macrophage-like RAW264.7 cells. ECC dose-dependently inhibited both the expression of iNOS protein and mRNA, resulting in decreased production of nitric oxide (NO), with an IC(50) of 80.5 mu g/mL. To investigate action mechanism by which ECC inhibits NO production and iNOS expression, we examined the activation of IkB in LPS-stimulated RAW264.7 cells. ECC clearly inhibited translocation of nuclear factor-kB (NF-kB) p65 subunits from cytosol to nucleus, which correlated with its inhibitory effects on IkB-alpha phosphorylation and degradation. Furthermore, ECC potently suppressed both the reporter gene expression and DNA-binding activity of NF-kB, which was associated with decreased p65 protein levels in the nucleus. Here, we show for the first time that ECC inhibits NF-kB activation through the inhibition of IkB degradation.
URI
http://hdl.handle.net/YU.REPOSITORY/25114http://dx.doi.org/10.3109/08923973.2010.520716
ISSN
0892-3973
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약학대학 > 약학부 > Articles
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