Ethylacetate fraction from Korean seaside starfish, Asterias amurensis, has an inhibitory effect on MMP-9 activity and expression and on migration behavior of TNF-alpha induced human aortic smooth muscle cells

Title
Ethylacetate fraction from Korean seaside starfish, Asterias amurensis, has an inhibitory effect on MMP-9 activity and expression and on migration behavior of TNF-alpha induced human aortic smooth muscle cells
Author(s)
장현욱서석종고현권[고현권]송권호[송권호]김정란[김정란]권경민[권경민]장영채[장영채]이영춘[이영춘]김동수[김동수]박성재[박성재]양주혜[양주혜]손종근나민균김철호[김철호]
Keywords
NF-KAPPA-B; MATRIX-METALLOPROTEINASE-9 EXPRESSION; MATRIX METALLOPROTEINASES; CULCITA-NOVAEGUINEAE; TISSUE INHIBITORS; LUIDIA-MACULATA; ARTERIAL INJURY; ACTIVATION; ATHEROSCLEROSIS; PATHOGENESIS
Issue Date
201106
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
TOXICOLOGY IN VITRO, v.25, no.4, pp.767 - 773
Abstract
Atherosclerosis is accompanied by the proliferation of human aortic smooth muscle cells (HASMC) and their movement into the intima. Many reports have indicated the involvement of gelatinases (MMP-9 and MMP-2) in this pathogenesis. The ethylacetate fraction from starfish, Asterias amurensis (EFA), harvested from the Korean seaside has an inhibitory effect on MMP-9 and MMP-2 activities, as well as on the expression of MMP-9 in TNF-alpha induced HASMC in a dose-dependent manner. Also, EFA inhibits the migration of TNF-alpha induced HASMC in transwells containing gelatin coated plugs. EFA was not cytotoxic to HASMC over the range 0-1 mg/ml. By Western-blot analysis, it was revealed that the phosphorylation of extracellular signal regulated kinase (ERK) in TNF-alpha induced cells was inhibited and nuclear factor kappa B (NF-kappa B) p65 levels in nuclear extracts were decreased by EFA treatment. In addition, ERK inhibitor (U0126) treated cells exhibited decreased MMP-9 activity in the zymographic assay. From these results, it was found that the gelatinolytic activity was regulated (1) by enzymatic inhibition of both MMP-9 and MMP-2, as well as (2) by the decreased production of MMP-9 via ERK pathways in EFA treated HASMCs. Taken together, it has been shown that EFA has a putative anti-atherosclerotic effect. (C) 2011 Elsevier Ltd. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/25112http://dx.doi.org/10.1016/j.tiv.2011.01.012
ISSN
0887-2333
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약학대학 > 약학부 > Articles
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