Silencing of the GnRH Type 1 Receptor Blocks the Antiproliferative Effect of the GnRH Agonist, Leuprolide, on the Androgen-independent Prostate Cancer Cell Line DU145

Title
Silencing of the GnRH Type 1 Receptor Blocks the Antiproliferative Effect of the GnRH Agonist, Leuprolide, on the Androgen-independent Prostate Cancer Cell Line DU145
Author(s)
고영휘Park, H. S.[Park, H. S.]Ha, Y. R[Ha, Y. R]Kim, J. W[Kim, J. W]Kang, S. G[Kang, S. G]Jang, H. A.[Jang, H. A.]Kang, S. H.[Kang, S. H.]Cheon, J.[Cheon, J.]
Keywords
GONADOTROPIN-RELEASING-HORMONE; MESSENGER-RNA; EXPRESSION; ANALOGS; INTERFERENCE; PROTEIN; LNCAP; PITUITARY; BIOLOGY; BINDING
Issue Date
201106
Publisher
FIELD HOUSE PUBLISHING LLP
Citation
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, v.39, no.3, pp.729 - 739
Abstract
This study investigated the mechanism of action of a gonadotropin-releasing hormone (GnRH) agonist, leuprolide, on proliferation of the hormone-refractory prostate cancer cell line DU145, transfected with short hairpin RNA (shRNA), to reduce expression of the GNRHR1 gene (which encodes the GnRH type 1 receptor). DU145 cell proliferation in the presence of leuprolide (10(-9) and 10(-7) M) or control medium was measured before and after GnRHR1 knockdown. Reverse transcription-polymerase chain reaction and Western blot analysis were used to measure the degree of GNRHR1 silencing. DU145 cells treated with leuprolide (10(-9) and 10(-7) M) showed significant growth inhibition compared with control-treated DU145 cells. Transfection with GNRHR1-shRNA significantly decreased GNRHR1 expression at 48 h. DU145 cells transfected with silencing GNRHR1-shRNA showed normal growth patterns; however, there was no significant inhibition of proliferation of DU145 cells transfected with GNRHR/-shRNA compared with cells transfected with control-shRNA in response to leuprolide. These data demonstrated that the antiproliferative effect of leuprolide was mediated by the GnRHR1.
URI
http://hdl.handle.net/YU.REPOSITORY/25071
ISSN
0300-0605
Appears in Collections:
의과대학 > 비뇨기과학교실 > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE