Characterization of IncF plasmids carrying the bla(CTX-M-14) gene in clinical isolates of Escherichia coli from Korea

Title
Characterization of IncF plasmids carrying the bla(CTX-M-14) gene in clinical isolates of Escherichia coli from Korea
Author(s)
이채훈김주원[김주원]배일권[배일권]정석훈[정석훈]장철훈[장철훈]이경원[이경원]
Keywords
SPECTRUM BETA-LACTAMASES; PREVALENCE; RESISTANCE; INTEGRON
Issue Date
201106
Publisher
OXFORD UNIV PRESS
Citation
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, v.66, no.6, pp.1263 - 1268
Abstract
Objectives: The purpose of this study was to investigate the molecular epidemiology of CTX-M-14-producing Escherichia coli clinical isolates from Korea. Methods: A total of 138 non-duplicate E. coli clinical isolates showing reduced susceptibility or resistance to ceftazidime and/or cefotaxime were included in the study. Resistance genes, genetic environment, R plasmid size and replicon type, sequence type (ST) and XbaI-macrorestriction patterns were determined. Results: Among 138 isolates, 35 were found to carry the bla(CTX-M-14) gene. The ISEcp1 element was identified in the upstream region of the bla(CTX-M-14) gene in 32 isolates. The bla(CTX-M-14) gene was located on an IncF plasmid in 21 isolates, on an IncA/C plasmid in 1 isolate, on the chromosome in 8 isolates and on both the chromosome and an IncF plasmid in 5 isolates. The most prevalent ST was ST405 (n=8), followed by ST354 (n=4), ST38 (n=3), ST69 (n=3) and the intercontinental ST, ST131 (n=3). PFGE and multilocus sequence typing experiments demonstrated no major clonal relationship among the CTX-M-14-producing isolates. Conclusions: The bla(CTX-M-14) gene was probably mobilized by IncF plasmids, which can readily spread in E. coli, causing horizontal dissemination of the resistance gene in Korea.
URI
http://hdl.handle.net/YU.REPOSITORY/25054http://dx.doi.org/10.1093/jac/dkr106
ISSN
0305-7453
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의과대학 > 진단검사의학교실 > Articles
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