Individual Variation in Growth Factor Concentrations in Platelet-rich Plasma and Its Influence on Human Mesenchymal Stem Cells

Title
Individual Variation in Growth Factor Concentrations in Platelet-rich Plasma and Its Influence on Human Mesenchymal Stem Cells
Author(s)
조희순송인환박소영성민철[성민철]안면환송경은[송경은]
Keywords
IN-VITRO; BONE-FORMATION; OSTEOGENIC DIFFERENTIATION; DERMAL FIBROBLASTS; PROGENITOR CELLS; PROLIFERATION; VIVO; PDGF; MIGRATION; GRAFTS
Issue Date
201107
Publisher
KOREAN SOC LABORATORY MEDICINE
Citation
KOREAN JOURNAL OF LABORATORY MEDICINE, v.31, no.3, pp.212 - 218
Abstract
Background: The objective of this study was to explore whether individual variations in the concentration of growth factors (GFs) influence the biologic effects of platelet-rich plasma (PRP) on human mesenchymal stem cells (HMSCs). Methods: The concentrations of 7 representative GFs in activated PRP (aPRP) were measured using ELISA. The effects of PRP on the proliferation and alkaline phosphatase (ALP) activity of HMSCs were examined using several concentrations of aPRP from 3 donors; the relationships between the GF levels and these biologic effects were then evaluated using 10% aPRP from 5 subgroups derived from 39 total donors. HMSCs were cultured in DMEM with the addition of aPRP for 4 or 12 days; then, DNA content and ALP activity were measured. Results: The quantity of DNA increased significantly at a 10% concentration of aPRP, but the ALP activity was suppressed at this concentration of aPRP. The GF concentrations varied among donors, and 5 subgroups of characteristic GF release patterns were identified via cluster analysis. DNA levels differed significantly between groups and tended to be higher in groups with higher concentrations of transforming growth factor-beta 1 (TGF-beta 1) and platelet-derived growth factors (PDGFs). DNA quantity was positively correlated with TGF-beta 1 concentration, and was negatively correlated with donor age. ALP activity was negatively correlated with PDGF-BB concentration. Conclusions: The varying GF concentrations may result in different biologic effects; thus, individual differences in GF levels should be considered for reliable interpretation of the biologic functions and standardized application of PRP.
URI
http://hdl.handle.net/YU.REPOSITORY/24892http://dx.doi.org/10.3343/kjlm.2011.31.3.212
ISSN
1598-6535
Appears in Collections:
의과대학 > 진단검사의학교실 > Articles
의과대학 > 해부학교실 > Articles
의과대학 > 생리학교실 > Articles
의과대학 > 정형외과학교실 > Articles
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