Batatasin I, a Naturally Occurring Phenanthrene Derivative, Isolated from Tuberous Roots of Dioscorea batatas Suppresses Eicosanoids Generation and Degranulation in Bone Marrow Derived-Mast Cells

Title
Batatasin I, a Naturally Occurring Phenanthrene Derivative, Isolated from Tuberous Roots of Dioscorea batatas Suppresses Eicosanoids Generation and Degranulation in Bone Marrow Derived-Mast Cells
Author(s)
손종근장현욱유예정미화박상조[박상조]손건호[손건호]
Keywords
C-KIT LIGAND; CYTOSOLIC PHOSPHOLIPASE A(2); ANTIOXIDANT ACTIVITIES; OCCUPATIONAL ASTHMA; STORAGE PROTEIN; CROSS-LINKING; 5-LIPOXYGENASE; EXPRESSION; COX-2; INHIBITION
Issue Date
201107
Publisher
PHARMACEUTICAL SOC JAPAN
Citation
BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.34, no.7, pp.1021 - 1025
Abstract
To find anti-inflammatory compounds from the tuberous roots of Dioscorea batatas, we isolated 6-hydroxy-2,4,7-trimethoxyphenanthrene (batatasin I) from the dichloromethane (CH2Cl2) fraction of this plant. Batatasin I inhibited both the generation of prostaglandin D-2 (PCD2), leukotriene C-4 (LTC4) and degranulation reaction in mouse bone marrow-derived mast cells (BMMCs). This compound inhibited cyclooxygenase-2 (COX-2) dependent PCD2 generation in a dose dependent manner, with IC50 values of 1.78 mu M. Western blotting probed with specific anti-COX-2 antibodies showed that the decrease in the quantity of the PGD(2) generation was accompanied by a decrease in the COX-2 protein level. In addition, this compound also inhibited the production of 5-lipoxygenase (5-LOX) dependent LTC4 in a dose dependent manner (IC50, 1.56 mu m). Batatasin I also inhibited the mast cell degranulation reaction (IC50 6.7 mu m) in BMMCs. This result indicates that batatasin I could be developed as an anti-inflammatory agent through further investigation.
URI
http://hdl.handle.net/YU.REPOSITORY/24888
ISSN
0918-6158
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약학대학 > 약학부 > Articles
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