RNA interference mediated knockdown of apolipophorin-III leads to knockdown of manganese superoxide dismutase in Hyphantria cunea

Title
RNA interference mediated knockdown of apolipophorin-III leads to knockdown of manganese superoxide dismutase in Hyphantria cunea
Author(s)
강영진서숙재[서숙재]하남규[하남규]전향미[전향미]김익수[김익수]한년수[한년수]이인희[이인희]진병래[진병래]김홍자[김홍자]권용민[권용민]김용일[김용일]
Keywords
DOUBLE-STRANDED-RNA; MITOCHONDRIAL PERMEABILITY TRANSITION; GALLERIA-MELLONELLA; HYDROGEN-PEROXIDE; IMMUNE-RESPONSES; LIPID TRANSPORT; MANDUCA-SEXTA; OXIDATIVE STRESS; CELL-DEATH; INSECT
Issue Date
201107
Publisher
ELSEVIER SCIENCE INC
Citation
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v.159, no.3, pp.303 - 312
Abstract
Apolipophorin-III(apoLp-III), a hemolymph protein that facilitates lipid transport in aqueous media in insects was recently shown to play a role in insect immune activation. Here, we report another novel possible function of apoLp-III in insects. To identify genes affected by apoLp-III expression in larvae, we decreased endogenous apoLp-III mRNA in Hyphantria cunea (Hc) through RNA interference; subsequently, we observed lower levels of antioxidant enzymes, including manganese superoxide dismutase (MnSOD), glutathione S-transferase, and immune proteins. Knockdown of Hc apoLp-III led to decreased MnSOD expression in fat body tissues and elevated superoxide anion levels in Hc fat body cells, suggesting that Hc apoLp-III is involved in the action and/or expression of antioxidant enzymes, especially MnSOD. (C) 2011 Elsevier Inc. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/24876http://dx.doi.org/10.1016/j.cbpa.2011.03.022
ISSN
1095-6433
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의과대학 > 약리학교실 > Articles
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