Luteolin-7-O-glucoside Suppresses Leukotriene C-4 Production and Degranulation by Inhibiting the Phosphorylation of Mitogen Activated Protein Kinases and Phospholipase C gamma 1 in Activated Mouse Bone Marrow-Derived Mast Cells

Title
Luteolin-7-O-glucoside Suppresses Leukotriene C-4 Production and Degranulation by Inhibiting the Phosphorylation of Mitogen Activated Protein Kinases and Phospholipase C gamma 1 in Activated Mouse Bone Marrow-Derived Mast Cells
Author(s)
장현욱김미화손건호[손건호]
Keywords
C-KIT LIGAND; CYTOKINE EXPRESSION; HUMAN NEUTROPHILS; MEDIATOR RELEASE; CROSS-LINKING; 5-LIPOXYGENASE; IGE; PROLIFERATION; BIOSYNTHESIS; ATHEROSCLEROSIS
Issue Date
201107
Publisher
PHARMACEUTICAL SOC JAPAN
Citation
BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.34, no.7, pp.1032 - 1036
Abstract
In this study, luteolin-7-O-glucoside (L7G), an herbal medicine isolated from Ailanthus altissima, inhibited 5-lipoxygenase (5-LOX)-dependent leukotriene C-4 (LTC4) production in bone marrow-derived mast cells (BMMCs) in a concentration-dependent manner with an IC50 of 3.0 mu M. To determine the action mechanism of L7G, we performed immunoblotting for cytosolic phospholipase A(2) (cPLA(2)) and mitogen-activated protein kinases (MAPKs) following c-kit ligand (KL)-induced activation of BMMCs with or without L7G. Inhibition of LTC4 production by L7G was accompanied by a decrease in cPLA(2) phosphorylation, which occurred via the extracellular signal-regulated protein kinase-1/2 (ERK1/2) and p38 and c-Jun N-terminal kinase (JNK) pathways. In addition, L7G also attenuated mast cell degranulation in a dose-dependent manner (IC50, 22.8 mu M) through inhibition of phospholipase C gamma 1 (PLC gamma 1) phosphorylation. Our results suggest that the anti-asthmatic activity of L7G may be mediated in part via the inhibition of LTC4 generation and mast cell degranulation.
URI
http://hdl.handle.net/YU.REPOSITORY/24873
ISSN
0918-6158
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약학대학 > 약학부 > Articles
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