Proteomic analysis of microvesicles derived from human colorectal cancer ascites

Title
Proteomic analysis of microvesicles derived from human colorectal cancer ascites
Author(s)
이경희최동식[최동식]박정옥[박정옥]장수철[장수철]윤예진[윤예진]정진우[정진우]최도영[최도영]김정욱[김정욱]강지선[강지선]박재성[박재성]황대희[황대희]박상현[박상현]김윤근[김윤근]Dominic M. Desiderio[Dominic M. Desiderio]김광표[김광표]고용송[고용송]
Keywords
MEMBRANE-VESICLES; OVARIAN-CANCER; MALIGNANT ASCITES; CELL-LINES; TUMOR; ANGIOGENESIS; EXOSOMES; EXPRESSION; EFFUSIONS; CARCINOMA
Issue Date
201107
Publisher
WILEY-BLACKWELL
Citation
PROTEOMICS, v.11, no.13, pp.2745 - 2751
Abstract
The presence of malignant ascites in the peritoneal cavity is a poor prognostic indicator of low survival rate. Various cancer cells, including those of colorectal cancer (CRC), release microvesicles (exosomes) into surrounding tissues and peripheral circulation including malignant ascites. Although recent progress has revealed that microvesicles play multiple roles in tumor progression, the protein composition and the pathological function of malignant ascites-derived microvesicles are still unknown. Here, we report the first global proteomic analyses of highly purified microvesicles derived from human CRC ascites. With 1-D SDS-PAGE and nano-LC-MS/MS analyses, we identified a total of 846 microvesicular proteins from ascites of three CRC patients with high confidence; 384 proteins were identified in at least two patients. We identified proteins that might function in tumor progression via disruption of epithelial polarity, migration, invasion, tumor growth, immune modulation, and angiogenesis. Furthermore, we identified several potential diagnostic markers of CRC including colon-specific surface antigens. Our proteomic analyses will help to elucidate diverse functions of microvesicles in cancer progression and will aid in the development of novel diagnostic tools for CRC.
URI
http://hdl.handle.net/YU.REPOSITORY/24871http://dx.doi.org/10.1002/pmic.201100022
ISSN
1615-9853
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의과대학 > 내과학교실 > Articles
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