Combination of Runx2 and BMP2 increases conversion of human ligamentum flavum cells into osteoblastic cells

Title
Combination of Runx2 and BMP2 increases conversion of human ligamentum flavum cells into osteoblastic cells
Author(s)
김재룡김현남[김현남]민우기[민우기]정재환[정재환]김성곤[김성곤]김신윤[김신윤]최제용[최제용]박병철[박병철]
Keywords
BONE MORPHOGENETIC PROTEIN-2; GROWTH-FACTOR BETA-1; MESENCHYMAL CELLS; GENE-EXPRESSION; UP-REGULATION; DIFFERENTIATION; C2C12; CBFA1; IMMORTALIZATION; MINERALIZATION
Issue Date
201107
Publisher
KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
Citation
BMB REPORTS, v.44, no.7, pp.446 - 451
Abstract
The conversion of fibroblasts into osteoblasts requires the activation of key signaling pathways, including the BMP pathway. Although Runx2 is known to be a component of the BMP pathway, the combination of Runx2 and BMP2 has not yet been examined with respect to the conversion of fibroblasts into osteoblasts. Here, human ligamentum flavum (LF) fibroblast-like cells from six patients were tested for their conversion into osteoblasts using adenoviruses expressing Runx2 or BMP2. The forced expression of Runx2 or BMP2 in primary cultured LF cells resulted in a variety of proliferation and differentiation behaviors. Combined treatment of BMP2 plus Runx2 resulted in better osteoblastic differentiation than treatment with either component alone. These results indicate that the Runx2 and BMP2 pathways possess both common and independent target genes. Collectively, Runx2 plus BMP2 mediated efficient conversion of fibroblast-like LF cells into osteoblast-like cells, suggesting the possible use of these components for clinical applications such as spinal fusion. [BMB reports 2011; 44(7): 446-451]
URI
http://hdl.handle.net/YU.REPOSITORY/24870http://dx.doi.org/10.5483/BMBRep.2011.44.7.446
ISSN
1976-6696
Appears in Collections:
의과대학 > 생화학.분자생물학교실 > Articles
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