Quantitative determination of daumone in rat plasma by liquid chromatography-mass spectrometry

Title
Quantitative determination of daumone in rat plasma by liquid chromatography-mass spectrometry
Author(s)
노금한[노금한]박정현[박정현]박정희[박정희]김민규[김민규]정만길[정만길]하헌주[하헌주]권광일[권광일]이화정[이화정]강원구
Keywords
NEMATODE CAENORHABDITIS-ELEGANS; DAUER-INDUCING PHEROMONE; CHEMOSENSORY NEURONS; LARVAL DEVELOPMENT; FOOD
Issue Date
201107
Publisher
ELSEVIER SCIENCE BV
Citation
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v.56, no.1, pp.114 - 117
Abstract
Daumone, 6-(3,5-dihydroxy-6-methyl-tetrahydro-pyran-2-yloxy)-heptanoic acid is a pheromone secreted by Caenorhabditis elegans, and has been known as a pivotal regulator of chemosensory processes in development and ageing. A quantification method using mass spectrometry was developed for the determination of daumone in rat plasma. After simple protein precipitation with acetonitrile including an internal standard, the analytes were chromatographed on a reversed-phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma daumone concentrations after a single intravenous administration of daumone in rats. (C) 2011 Elsevier B.V. All rights reserved.
URI
http://hdl.handle.net/YU.REPOSITORY/24863http://dx.doi.org/10.1016/j.jpba.2011.04.008
ISSN
0731-7085
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약학대학 > 약학부 > Articles
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