A bHLH transcription factor, DvIVS, is involved in regulation of anthocyanin synthesis in dahlia (Dahlia variabilis)

Title
A bHLH transcription factor, DvIVS, is involved in regulation of anthocyanin synthesis in dahlia (Dahlia variabilis)
Author(s)
박경일Sho Ohno[Sho Ohno]Munetaka Hosokawa[Munetaka Hosokawa]Atsushi Hoshino[Atsushi Hoshino]Yoshikuni Kitamura[Yoshikuni Kitamura]Yasumasa Morita[Yasumasa Morita]Akiko Nakashima[Akiko Nakashima]Ayumi Deguchi[Ayumi Deguchi]Fumi Tatsuzawa[Fumi Tatsuzawa]Motoaki Doi[Motoaki Doi]Shigeru Iida[Shigeru Iida]Susumu Yazawa[Susumu Yazawa]
Keywords
JAPANESE MORNING GLORY; TRANSPOSABLE ELEMENT; MOLECULAR CHARACTERIZATION; FLAVONOID GLYCOSIDES; FLOWER PIGMENTATION; VARIEGATED FLOWERS; ANTIRRHINUM-MAJUS; CHALCONE SYNTHASE; PETUNIA ENCODES; GERBERA-HYBRIDA
Issue Date
201107
Publisher
OXFORD UNIV PRESS
Citation
JOURNAL OF EXPERIMENTAL BOTANY, v.62, no.14, pp.5105 - 5116
Abstract
Dahlias (Dahlia variabilis) exhibit a wide range of flower colours because of accumulation of anthocyanin and other flavonoids in their ray florets. Two lateral mutants were used that spontaneously occurred in 'Michael J' (MJW) which has yellow ray florets with orange variegation. MJOr, a bud mutant producing completely orange ray florets, accumulates anthocyanins, flavones, and butein, and MJY, another mutant producing completely yellow ray florets, accumulates flavones and butein. Reverse transcription-PCR analysis showed that expression of chalcone synthase 1 (DvCHS1), flavanone 3-hydroxylase (DvF3H), dihydroflavonol 4-reductase (DvDFR), anthocyanidin synthase (DvANS), and DvIVS encoding a basic helix-loop-helix transcription factor were suppressed, whereas that of chalcone isomerase (DvCHI) and DvCHS2, another CHS with 69% nucleotide identity with DvCHS1, was not suppressed in the yellow ray florets of MJY. A 5.4 kb CACTA superfamily transposable element, transposable element of Dahlia variabilis 1 (Tdv1), was found in the fourth intron of the DvIVS gene of MJW and MJY, and footprints of Tdv1 were detected in the variegated flowers of MJW. It is shown that only one type of DvIVS gene was expressed in MJOr, whereas these plants are likely to have three types of the DvIVS gene. On the basis of these results, the mechanism regulating the formation of orange and yellow ray florets in dahlia is discussed.
URI
http://hdl.handle.net/YU.REPOSITORY/24850http://dx.doi.org/10.1093/jxb/err216
ISSN
0022-0957
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자연자원대학 > 원예생명과학과 > Articles
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