Pmgeny Analysis and Selection of Tomato Transformants with patII Gene linked to Inherent Disease Resistance Gene

Title
Pmgeny Analysis and Selection of Tomato Transformants with patII Gene linked to Inherent Disease Resistance Gene
Author(s)
안순영강권규[강권규]윤해근박효근[박효근]
Keywords
AGROBACTERIUM-TUMEFACIENS; TRANSGENE BEHAVIOR; PLANTS; INOCULATION; SYSTEM; MAIZE; DNA
Issue Date
201108
Publisher
KOREAN SOC HORTICULTURAL SCIENCE
Citation
KOREAN JOURNAL OF HORTICULTURAL SCIENCE & TECHNOLOGY, v.29, no.4, pp.345 - 351
Abstract
This study was carried out to develop a model system using selection method for disease resistant plant breeding programs using a herbicide bialaphos-resistant patII gene as a gene-based marker. Spraying bialaphos could eliminate the susceptible plants from the segregating populations such as F(2)'s and thereafter. Tomato cv. Momotaro-yoke was transformed with patII gene 60 independent transformants were acquired. Total 42 transformants were analyzed in transgene copy numbers by Southern blotting and the segregation ratios for the bialaphos resistance. Statistical analysis revealed that the transgene copy numbers and the segregation ratios were not always coincided, especially having the tendency of underestimating the real numbers of the transgenes in the multicopy lines. A two-stepwise screening method was applied to select T(1) tomato plants which linked the transgenic patII to a disease resistance gene (I2 and Ve). Based on the resistant to susceptible ratios, T-20 plant was finally selected due to the estimated linkage 12-13 cM between the patII gene to the I2 gene on chromosome 11. This newly developed system could be applied to any economical crop in breeding programs.
URI
http://hdl.handle.net/YU.REPOSITORY/24772
ISSN
1226-8763
Appears in Collections:
중앙도서관 > rims journal
자연자원대학 > 원예생명과학과 > Articles
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