저온 환경에서 Cronobacter sakazakii의 저항과 생존

Title
저온 환경에서 Cronobacter sakazakii의 저항과 생존
Other Titles
Resistance and Survival of Cronobacter sakazakii under Environmental Stress of Low Temperature
Author(s)
방우석김세훈[김세훈]장성란[장성란]정현정[정현정]
Keywords
Cronobacter sakazakii; cold environment; response of cold adaptation; freeze-thaw resistance; Cronobacter sakazakii; cold environment; response of cold adaptation; freeze-thaw resistance
Issue Date
201108
Publisher
한국식품저장유통학회
Citation
한국식품저장유통학회지, v.18, no.4, pp.612 - 619
Abstract
Cronobacter sakazakii has been isolated from a wide range of environmental sources and from several foods of animal and plant origin. The objective of this study was to determine the resistance of C. sakazakii (ATCC 12868, ATCC 29004, and ATCC 29544) in cold, cold-freeze thaw, cold-acid, and cold starvation-freeze thaw stress. The number of C. sakazakii decreased to 1 log CFU/mL at 5℃ (cold storage) for 10 days. When C. sakazakii was cultivated at a low temperature (13℃), the population of C. sakazakii ATCC 12868 and 29004 increased to 10⁹ CFU/mL, and the population of C. sakazakii ATCC 29544 increased to 10⁸ CFU/mL. For C. sakazakii ATCC 12868 and 29004, the cold-adapted cells (5℃ 24 hr) decreased by 4 log CFU/mL, and the low-temperature-cultivated cells (13℃) decreased by 0.5 log CFU/mL. In this study, low-temperature cultivation enhanced the freeze-thaw cross-resistance due to the metabolic changes in the cells. Cold stress (5℃ 48 hr, 13℃ cultivation) enhanced the cold-acid cross-resistance. The cold-starved cells in the sterilized 0.1% peptone water enhanced the freeze-thaw cross-resistance with significant differences (p<0.05). Therefore, the increased tolerance of the cold-adapted or low-temperature-cultivated C. sakazakii cells to freeze-thaw, acid, or starvation suggests that such environments should be considered when processing minimally processed foods or foods with extended shelf life.
URI
http://hdl.handle.net/YU.REPOSITORY/24660
ISSN
1738-7248
Appears in Collections:
생활과학대학 > 식품영양학과 > Articles
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